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Proceedings Paper

Ho:YAG laser: intervertebral disk cell interaction using three-dimensional cell culture system
Author(s): Masato Sato; Miya Ishihara; Tsunenori Arai; Takashi Asazuma; Toshiyuki Kikuchi; Makoto Kikuchi; Kyosuke Fujikawa
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Paper Abstract

The purpose of this study is to evaluate the influence on the intervertebral disc cells after laser irradiation using three- dimensional culture system and to clarify the optimum Ho:YAG laser irradiation condition on percutaneous laser disc decompression (PLDD) for lumbar disc herniation. Since the Ho:YAG laser ablation is characterized by water-vapor bubble dynamics, not only thermal effect but also acoustic effect on cell metabolism might occur in the intervertebral disc. We studied the disc cell reaction from the metabolic point of view to investigate photothermal and photoacoustic effects on three-dimensional cultured disc cell. Intervertebral discs were obtained from female 30 Japanese white rabbits weighing about 1 kg. A pulsed Ho:YAG laser (wavelength: 2.1 micrometer, pulse width: about 200 microseconds) was delivered through a 200 micrometer-core diameter single silica glass fiber. We used the Ho:YAG laser irradiation fluence ranging from 60 to approximately 800 J/cm2 at the fiber end. To investigate acoustic effect, the acoustic transducer constructed with polyvinylidene fluoride (PVdF) film and acoustic absorber was used to detect the stress wave. Thermocouple and thermography were used to investigate thermal effect. Concerning damage of plasma membrane and ability of matrix synthesis, thermal effect might mainly affect cell reaction in total energy of 54 J (closed to practically used condition), but in 27 J, acoustic effect might contribute to it. We found that total energy was key parameter among the optimum condition, so that temperature and/or stress wave may influence Ho:YAG laser-disc cell interactions.

Paper Details

Date Published: 13 June 2000
PDF: 6 pages
Proc. SPIE 3914, Laser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical, (13 June 2000); doi: 10.1117/12.388048
Show Author Affiliations
Masato Sato, National Defense Medical College (Japan)
Miya Ishihara, National Defense Medical College (Japan)
Tsunenori Arai, National Defense Medical College (Japan)
Takashi Asazuma, National Defense Medical College (Japan)
Toshiyuki Kikuchi, National Defense Medical College (Japan)
Makoto Kikuchi, National Defense Medical College (Japan)
Kyosuke Fujikawa, National Defense Medical College (Japan)

Published in SPIE Proceedings Vol. 3914:
Laser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical
Jeffrey O. Hollinger D.D.S.; Donald Dean Duncan; Jeffrey O. Hollinger D.D.S.; Donald Dean Duncan; Steven L. Jacques, Editor(s)

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