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Proceedings Paper

Functional imaging of single cells with photothermal microscopy
Author(s): Dmitry Lapotko
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Paper Abstract

Photothermal technique for light microscopy is reported as the new tool for quantitative analysis of single living cell structural and functional properties. Probe laser illumination with phase contrast method allows to visualize thermal field being induced in cell due to absorption of pump laser radiation. In our set up the cell is pumped by the pulsed laser at 532 nm, 10 ns, 0.01-0.4 mJ. The source of probe beam is a pulsed dye laser which forms cell image. Fully automated image cytometer with acquisition rate 1cell/s is described. An acquired cell image is considered as spatially and temporally resolved cell response to non- specific load being induced with a pump laser. Thus we introduce photothermal image cytometry which may be applied for investigation of mechanisms of photodamage, single cell dosimetry, cell functions and structure and thermal phenomena at cell level.

Paper Details

Date Published: 19 May 2000
PDF: 10 pages
Proc. SPIE 3916, Biomedical Optoacoustics, (19 May 2000); doi: 10.1117/12.386330
Show Author Affiliations
Dmitry Lapotko, Luikov Heat and Mass Transfer Institute (United States)

Published in SPIE Proceedings Vol. 3916:
Biomedical Optoacoustics
Alexander A. Oraevsky, Editor(s)

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