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Proceedings Paper

Fluorescence detection of oral squamous cell carcinoma using Hyperflav
Author(s): Ivan S. Melnik; Sergiy M. Dets; Andrew H. Rawicz; Lewei Zhang
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Paper Abstract

A novel hypericin-based drug HyperflavTM has been evaluated for light-induced fluorescence detection of oral cancer. Squamous cell carcinoma was induced with carcinogenic agent in right pouches of forty hamsters (20/20 males/females). Solution of HyperflavTM was sprinkled into stomach with a single dose 0.2 - 4 mg of pure hypericin per kg b.w. and 4 - 8 hours before fluorescence analysis. In two animal groups with cancer symptoms the autofluorescence and hypericin-induced fluorescence were taken under 442 nm excitation. The buccal mucosa and adjacent areas were measured fiberoptically in-vivo and in-vitro using orange/green ratio (610/540). The in-vivo fluorescence imaging of malignant areas was conducted to assist the biopsy guidance and to compare with white-light images. Histological and morphological analyses were performed from biopsies. Oral squamous cell carcinoma in its early stage demonstrated specific higher 610/540 ratio for 37 tested hamsters. Advanced state involved another higher fluorescence maximum around 640 nm that in our opinion caused by strong porphyrin-induced native fluorescence. Such deformation of fluorescence spectra may lead to inadequate perception of diseased tissue area. To avoid this problem the autofluorescence spectra & images were added. HyperflavTM application is promising for demarcation of early oral cancer when combined with autofluorescence measurements.

Paper Details

Date Published: 17 May 2000
PDF: 6 pages
Proc. SPIE 3907, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems X, (17 May 2000); doi: 10.1117/12.386269
Show Author Affiliations
Ivan S. Melnik, Simon Fraser Univ. (Canada)
Sergiy M. Dets, Simon Fraser Univ. and National Technical Univ. of Ukraine (Canada)
Andrew H. Rawicz, Simon Fraser Univ. (Canada)
Lewei Zhang, Univ. of British Columbia (Canada)

Published in SPIE Proceedings Vol. 3907:
Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems X
R. Rox Anderson M.D.; Kenton W. Gregory M.D.; Eugene A. Trowers M.D.; David S. Robinson M.D.; Kenneth Eugene Bartels D.V.M.; Lou Reinisch; Reza S. Malek M.D.; C. Gaelyn Garrett M.D.; Lloyd P. Tate V.D.M.; Hans-Dieter Reidenbach; Timothy A. Woodward M.D.; Kenneth Eugene Bartels D.V.M.; Lawrence S. Bass M.D.; George M. Peavy D.V.M.; C. Gaelyn Garrett M.D.; Kenton W. Gregory M.D.; Nikiforos Kollias; Harvey Lui M.D.; Reza S. Malek M.D.; George M. Peavy D.V.M.; Hans-Dieter Reidenbach; Lou Reinisch; David S. Robinson M.D.; Lloyd P. Tate V.D.M.; Eugene A. Trowers M.D.; Timothy A. Woodward M.D., Editor(s)

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