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Proceedings Paper

Visible spectroscopic imaging studies of normal and ischemic dermal tissue
Author(s): Karel J. Zuzak; Michael D. Schaeberle; E. Neil Lewis; Ira W. Levin
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Paper Abstract

We describe a non-invasive, in vivo hyperspectral imaging method for visualizing the spatial distribution of dermal tissue oxygenation. Real-time images of the dermis are acquired both at multiple, contiguous wavelengths and at relatively narrow spectral bandwidths to generate a data cube consisting of one spectral and two spatial dimensions. For data collection, the sample area is illuminated by radiation, which is delivered by liquid light guides from a quartz tungsten halogen source. Reflected light from the sample is first passed through a liquid crystal tunable filter and then imaged onto a silicon charged coupled device detector. The subsequently digitized data are presented in terms of spectral images reflecting multivariate least squares analyses based upon linear combinations of oxy- and deoxyhemoglobin reference spectra. The generated gray scale images directly represent the varying spatial distributions of dermal tissue oxygenation. As an example, imaging data are obtained from normal tissue and induced ischemic tissue for which both the venous and arterial blood flow was artificially occluded.

Paper Details

Date Published: 8 May 2000
PDF: 10 pages
Proc. SPIE 3918, Biomedical Spectroscopy: Vibrational Spectroscopy and Other Novel Techniques, (8 May 2000); doi: 10.1117/12.384958
Show Author Affiliations
Karel J. Zuzak, National Institutes of Health (United States)
Michael D. Schaeberle, National Institutes of Health (United States)
E. Neil Lewis, Spectral Dimensions, Inc. (United States)
Ira W. Levin, National Institutes of Health (United States)

Published in SPIE Proceedings Vol. 3918:
Biomedical Spectroscopy: Vibrational Spectroscopy and Other Novel Techniques
Anita Mahadevan-Jansen; Gerwin J. Puppels, Editor(s)

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