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Proceedings Paper

Rapid quantification of the clinically important analytes in sub-ul simulating human sera
Author(s): Jianan Y. Qu; Lan Shao
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Paper Abstract

Near IR Raman spectroscopy has been successfully used to analyze major metabolites in simulating human serum samples quantitatively. We demonstrated that the optical waveguide based Raman cell and signal collector can significantly improve the collection efficiency of inherently weak Raman signal. The acquisition time for the Raman signal with decent SNR has been reduced to 10 seconds. The sample container, a quartz capillary with length of 20 mm and diameter of 400 micrometers , is easy to produce and disposable. The Raman signal was normalized to the dominant water peak at 3350 cm-1. This self-calibration method makes the measurement insensitive to the fluctuation of the excitation and misalignment of the collection system. A partial least squares method was used to predict the analyte concentrations of interest in the simulating human sera. The prediction accuracy of albumin, globulin, triactin, urea and glucose is greatly acceptable for clinical diagnostics. The results of this research encourage the development of practical Raman system for clinical diagnostics.

Paper Details

Date Published: 8 May 2000
PDF: 7 pages
Proc. SPIE 3918, Biomedical Spectroscopy: Vibrational Spectroscopy and Other Novel Techniques, (8 May 2000); doi: 10.1117/12.384938
Show Author Affiliations
Jianan Y. Qu, Hong Kong Univ. of Science and Technology (Hong Kong)
Lan Shao, Hong Kong Univ. of Science and Technology (Hong Kong)

Published in SPIE Proceedings Vol. 3918:
Biomedical Spectroscopy: Vibrational Spectroscopy and Other Novel Techniques
Anita Mahadevan-Jansen; Gerwin J. Puppels, Editor(s)

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