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Proceedings Paper

Kinetic adhesion of antibody to a silicon wafer studied by laser reflectometry
Author(s): Bibiana Doris Riquelme; Sebastian P. Rius; Veronica V. Bozza; Juana R. Valverde; Rodolfo Jose Rasia
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Paper Abstract

Study of biomolecular interaction dynamics and relations between their structure and function is very important for the understanding of biological system. The laser diffractometry technique, as well as the surface plasmon resonance technique, can give information about the interaction kinetics, molecular binding stoichiometry, and the concentration of molecules in a solution. This method offers detailed and accurate determination of real-time adsorption kinetics of protein without labeling of the protein. When the polarized light is reflected on a solid surface, the reflectance has a minimum at the principal incidence angle. As the proteins are adsorbed over the surface the reflectance increases. Therefore, the temporal register of reflected intensity gives curves representing the binding kinetics of antibody in real-time. The technique is highly sensible because of the great difference in the refractive index between silicon and organic material. This method was applied to study the adhesion kinetics of monoclonal anti-AB antibodies at surface of a silicon wafer. The mathematical analysis of results would suggest that the antibody association occurs according to classical Langmuir surface adsorption. This analysis permits to obtain the adhesion and dissociation of the antibody.

Paper Details

Date Published: 3 May 2000
PDF: 8 pages
Proc. SPIE 3911, Biomedical Diagnostic, Guidance, and Surgical-Assist Systems II, (3 May 2000); doi: 10.1117/12.384923
Show Author Affiliations
Bibiana Doris Riquelme, Univ. Nacional de Rosario (Argentina)
Sebastian P. Rius, Univ. Nacional de Rosario (Argentina)
Veronica V. Bozza, Univ. Nacional de Rosario (Argentina)
Juana R. Valverde, Univ. Nacional de Rosario (Argentina)
Rodolfo Jose Rasia, Univ. Nacional de Rosario (Argentina)

Published in SPIE Proceedings Vol. 3911:
Biomedical Diagnostic, Guidance, and Surgical-Assist Systems II
Tuan Vo-Dinh; Warren S. Grundfest M.D.; David A. Benaron M.D., Editor(s)

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