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Proceedings Paper

One-step conjugation of biomolecules to luminescent nanocrystals
Author(s): Warren C. W. Chan; Tara L. Prendergast; Manas Jain; Shuming Nie
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Paper Abstract

The development of optical pulses is crucial to the elucidation of complex biological and chemical problems. Existing fluorescent labels tend to photobleach, have low luminescence, and area sensitive to the environment. These problems can be overcome by the use of semiconductor quantum dots. These ZnS-capped CdSe quantum dots are synthesized in an organic media. To use them for biological labeling, the quantum dots are made water-soluble by adsorbing bifunctional molecules onto the surface. This step is crucial for the successful conjugation of biomolecules onto the surface. In this report, a new method for the direct adsorption of biomolecules onto the surface of quantum dots is demonstrated. Biomolecules such as glutathione, mercaptusuccic acid, and histidine are directly conjugated to luminescent quantum dots by S-Zn or N-Zn bonding.

Paper Details

Date Published: 26 April 2000
PDF: 8 pages
Proc. SPIE 3924, Molecular Imaging: Reporters, Dyes, Markers, and Instrumentation, (26 April 2000); doi: 10.1117/12.384243
Show Author Affiliations
Warren C. W. Chan, Indiana Univ. (United States)
Tara L. Prendergast, Indiana Univ. (United States)
Manas Jain, Indiana Univ. (United States)
Shuming Nie, Indiana Univ. (United States)

Published in SPIE Proceedings Vol. 3924:
Molecular Imaging: Reporters, Dyes, Markers, and Instrumentation
Darryl J. Bornhop; Kai Licha, Editor(s)

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