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Proceedings Paper

Multiparameter breast cancer cell image analysis for objective estimation of nuclear grade: comparison with light microscopic observational data
Author(s): Juris Berzins; Uldis Sneiders; Daina Plegere; Talivaldis Freivalds; Romalda Grigalinovica
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Paper Abstract

We performed a multi parameter image analysis assessment of breast cancer cell population nuclear grade (NG), which is regarded as one of the main prognostic factors for treatment efficacy and survival of the patients and compared it with light microscopic estimation of NG. Cytological imprint slides from 20 ductal carcinomas were stained according to Leischmann-AzureII-eosine method, and NG was estimated by light microscopic observation according to Black in Fisher's modification. Simultaneously, using specially elaborated software, in each patient 100 cancer cells were analyzed for nuclear perimeter, diameter, area, nucleolar area, and average intensity of staining. The chromatin structure was assessed using mean diameter of chromatin grains and relatively chromatic are within the nucleus. Light microscopic estimation revealed 4/15 grade 2 and 7/15 grade 3 tumors out of 15 filtrating ductal carcinomas, with 4/15 classified as intermediate between grade 2-3. Multifactoral linear correlation coefficient r equals 0.39, p < 0.001 for ductal cancer, higher NG comes with increasing nucleolar area, nuclear roundness factor, nuclear are, and chromatin area within the cell nucleus. Image analysis may yield precise information on NG as a prognostic factor in breast cancer patients.

Paper Details

Date Published: 27 April 2000
PDF: 6 pages
Proc. SPIE 3921, Optical Diagnostics of Living Cells III, (27 April 2000); doi: 10.1117/12.384207
Show Author Affiliations
Juris Berzins, Latvian Cancer Ctr. (Latvia)
Uldis Sneiders, Latvian Cancer Ctr. (Latvia)
Daina Plegere, Latvian Cancer Ctr. (Latvia)
Talivaldis Freivalds, Institute of Experimental and Clinical Medicine/Univ. of Latvia (Latvia)
Romalda Grigalinovica, P. Stradins Univ. Hospital (Latvia)

Published in SPIE Proceedings Vol. 3921:
Optical Diagnostics of Living Cells III
Daniel L. Farkas; Daniel L. Farkas; Robert C. Leif, Editor(s)

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