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Proceedings Paper

Probing single-molecule orientations in confined sample regions with near-field scanning optical microscopy
Author(s): Christopher W. Hollars; Robert C. Dunn
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Paper Abstract

Near-field scanning optical microscopy (NSOM) is utilized to study the 3D orientation of fluorescent probe molecules doped into thin polymer films and lipid monolayers. A simplified model of the fields near the NSOM tip aperture is used to simulate the single molecule near-field fluorescence features and extract the orientation of the probe molecules in the films. These measurements are particular useful for semi-ordered systems such as lipid monolayers that are often utilized as models of biological membranes. These single molecule measurements can reveal new features and provide details previously hidden in bulk methods that average over large ensembles of molecules.

Paper Details

Date Published: 21 April 2000
PDF: 5 pages
Proc. SPIE 3922, Scanning and Force Microscopies for Biomedical Applications II, (21 April 2000); doi: 10.1117/12.383350
Show Author Affiliations
Christopher W. Hollars, Univ. of Kansas (United States)
Robert C. Dunn, Univ. of Kansas (United States)

Published in SPIE Proceedings Vol. 3922:
Scanning and Force Microscopies for Biomedical Applications II
Shuming Nie; Eiichi Tamiya; Edward S. Yeung, Editor(s)

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