Share Email Print

Proceedings Paper

Single molecule probes of lipid membrane dynamics
Author(s): Chad E. Talley; Robert C. Dunn
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

Single molecule fluorescence measurements are used to characterize the local dynamics in model lipid films of DPPC. Analysis of emission trajectory autocorrelations reveals a dependence in the single molecule emission fluctuations which is correlated with the surface pressure of the lipid monolayer. Comparison of DPPC monolayers transferred onto mica at (pi) equals 5 mN/m and (pi) equals 30 mN/m exhibit characteristic single molecule fluctuation times of 700 ms and 1.30 s, respectively. These fluctuation times are correlated with the order in the film. Comparison with recent near-field measurements of probe molecule orientation and AFM measurements of film topography, however, indicate that the lipid phase surrounding the probe molecule remains the same from (pi) equals 5 mN/m to 30 mN/m. The increase in the characteristic emission fluctuation times with increasing surface pressure, therefore, reflects a decrease in the freedom surrounding a single molecule. These fluctuations are consistent with long range concerted motions of the lipid tailgroups in the lipid film.

Paper Details

Date Published: 21 April 2000
PDF: 4 pages
Proc. SPIE 3922, Scanning and Force Microscopies for Biomedical Applications II, (21 April 2000); doi: 10.1117/12.383346
Show Author Affiliations
Chad E. Talley, Univ. of Kansas (United States)
Robert C. Dunn, Univ. of Kansas (United States)

Published in SPIE Proceedings Vol. 3922:
Scanning and Force Microscopies for Biomedical Applications II
Shuming Nie; Eiichi Tamiya; Edward S. Yeung, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?