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Proceedings Paper

Quantitative fluorescence spectroscopy of single molecules on surfaces
Author(s): Martin Boehmer; Joerg Enderlein; Stefan Seeger
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Paper Abstract

In recent years, laser induced fluorescence detection and spectroscopy of single molecules (SMD) has seen a tremendous development. Besides fundamental research on individual molecular systems, practical applications of this sensitive detection technique for analytical and diagnostic purposes are becoming more and more important. For a wider applicability of the SMD technique it is desirable to detect not only the presence or absence of a molecule within a given detection volume,, but also t be able to quantify this fluorescence absolutely. Modified flow cytometry system for SMD that are ensuring such a quantifiable fluorescence detection of single molecules are already successfully applied for e.g. DNA fragment sizing. In our talk, we present a modified confocal detection set-up for performing SMD on surfaces that aims at a quantified detection of single molecule fluorescence. First experimental results presented and emerging problems are discussed.

Paper Details

Date Published: 21 April 2000
PDF: 7 pages
Proc. SPIE 3922, Scanning and Force Microscopies for Biomedical Applications II, (21 April 2000); doi: 10.1117/12.383339
Show Author Affiliations
Martin Boehmer, Univ. Regensburg (Germany)
Joerg Enderlein, Univ. Regensburg (Germany)
Stefan Seeger, Zurich Univ. (Switzerland)

Published in SPIE Proceedings Vol. 3922:
Scanning and Force Microscopies for Biomedical Applications II
Shuming Nie; Eiichi Tamiya; Edward S. Yeung, Editor(s)

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