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Proceedings Paper

High-throughput single-molecule DNA screening based on electrophoresis
Author(s): Michael R. Shortreed; Hanlin Li; Edward S. Yeung
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Paper Abstract

In disease diagnosis, DNA and proteins are distinguished based on charge and hydrodynamic radius. Many protein assays and DNA assays relevant to cancer diagnosis are based on electrophoresis. However, standard protocols are not only slow but also insensitive. We successfully demonstrated a high-throughput imaging approach that allows determination of the individual electrophoretic mobilities of many molecules at a time. Each measurement on ly requires a few ms to compete. This opens up the possibility of screening single copies of DNA or proteins within single biological cells for disease markers without performing polymerase chain reaction or other biological amplification.

Paper Details

Date Published: 21 April 2000
PDF: 9 pages
Proc. SPIE 3922, Scanning and Force Microscopies for Biomedical Applications II, (21 April 2000); doi: 10.1117/12.383331
Show Author Affiliations
Michael R. Shortreed, Iowa State Univ. (United States)
Hanlin Li, Iowa State Univ. (United States)
Edward S. Yeung, Iowa State Univ. (United States)

Published in SPIE Proceedings Vol. 3922:
Scanning and Force Microscopies for Biomedical Applications II
Shuming Nie; Eiichi Tamiya; Edward S. Yeung, Editor(s)

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