We have used the cross-polarization method to eliminate the specular reflection and enhance the diffusive back- scattering of polarized fluorescence excitation light from the turbid media. The image of the ratio between fluorescence and cross-polarized reflection provides a map of the fluorescence yield, define das the ratio of the autofluorescence emission to the illumination incidence, over the surface of the turbid media when excitation and collection of fluorescence are highly inhomogeneous. The simple ratio imaging technique shows the feasibility to detect early cancer, which usually starts from the superficial layer of tissue, based on the contrast in the fluorescence yield between lesion and normal tissue.

Date Published: 19 April 2000
PDF: 6 pages
Proc. SPIE 3917, Optical Biopsy III, (19 April 2000); doi: 10.1117/12.382749

Published in SPIE Proceedings Vol. 3917:
Optical Biopsy III
Robert R. Alfano, Editor(s)