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Proceedings Paper

Characterization of human neoplastic and normal oral tissues by visible excitation and emission fluorescence spectroscopy
Author(s): D. Koteeswaran; Nammalver Vengadesan; Prakasa Rao Aruna; K. Muthuvelu; S. Bharghavi; V. S. Gowri; Singaravelu Ganesan
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Paper Abstract

The steady state native fluorescence emission and excitation spectra of human normal and cancerous oral tissues are studied in the visible region. The fluorescence excitation spectrum is recorded for 600 nm emission by scanning the excitation. The excitation spectrum of normal tissues has peaks at 406, 524 and 552 nm, whereas the cancerous tissues have peaks at 406, 513 and 552 nm respectively. The fluorescence emission spectra were also recorded at 405 and 560 nm excitations. The emission spectrum of cancerous tissues has two distinct peaks at 604 and 660 nm. It is also observed that there is a distinct difference between normal and cancerous tissues at 560 nm excitation. The ratio parameter R1$ equals (I(subscript 406/I550) is introduced from the excitation spectrum for 600 nm emission and two ratio parameters R2 equals (I470/I600) and R3 equals (I470/I660) are introduced for the emission spectrum at 405 nm excitation. Among the three ratio parameters the R1 classifies the normal and cancerous tissues at a specificity and sensitivity of 83 percent and 93 percent respectively. A critical value of 1.8 is suggested for classifying the normal from cancerous tissues.

Paper Details

Date Published: 19 April 2000
PDF: 5 pages
Proc. SPIE 3917, Optical Biopsy III, (19 April 2000); doi: 10.1117/12.382745
Show Author Affiliations
D. Koteeswaran, Government Aringnar Anna Memorial Cancer Hospital (India)
Nammalver Vengadesan, Anna Univ. (India)
Prakasa Rao Aruna, Anna Univ. (India)
K. Muthuvelu, Government Aringnar Anna Memorial Cancer Hospital (India)
S. Bharghavi, Government General Hospital/Chennai Medical College (India)
V. S. Gowri, Anna Univ. (India)
Singaravelu Ganesan, Anna Univ. (India)

Published in SPIE Proceedings Vol. 3917:
Optical Biopsy III
Robert R. Alfano, Editor(s)

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