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Proceedings Paper

Immunoaffinity-based phosphorescent sensor platform for the detection of bacterial spores
Author(s): Peter F. Scholl; C. Brent Bargeron; Terry E. Phillips; Tommy Wong; Sala Abubaker; John D. Groopman; Paul T. Strickland; Richard C. Benson
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Paper Abstract

Consideration of emergency response plans to an attack with biological weapons such as anthrax spores has spawned renewed interest in the development of inexpensive, rapid, and sensitive field portable sensors for use by non- specialists. The conceptual feasibility of such a device is demonstrated via the immunoaffinity capture of spores of the anthrax simulant B. globigii on a column followed by their washing, elution and phosphorescent detection. Spores are generically detected via the rapid extraction of dipicolinic acid (DPA) followed by its chelation with terbium to yield a phosphorescent complex. Chemical, thermal and mechanical methods of DPA extraction were evaluated. Simple extraction in HNO3 released up to 5 percent of the spore weight as DPA within 60 seconds. Extraction in H2O liberated 7 percent of the spore weight as DPA. Sonication with glass beads in H2O for 45 seconds released up to 4 percent of the spore weight as DPA. It is estimated that implementation of these techniques will permit development of a device requiring 3-5 minutes per analysis with a limit of detection on the order of 500 ng spore/mL. This approach is not intended to replace more specific methods of analysis. However, it is proposed for consideration as an inexpensive, simple and rapid means of spore detection by non-specialists in emergency situations.

Paper Details

Date Published: 11 April 2000
PDF: 11 pages
Proc. SPIE 3913, In-Vitro Diagnostic Instrumentation, (11 April 2000); doi: 10.1117/12.382033
Show Author Affiliations
Peter F. Scholl, Johns Hopkins Univ. (United States)
C. Brent Bargeron, Johns Hopkins Univ. (United States)
Terry E. Phillips, Johns Hopkins Univ. (United States)
Tommy Wong, Army Research Lab. (United States)
Sala Abubaker, Johns Hopkins Univ. School of Hygiene and Public Health (United States)
John D. Groopman, Johns Hopkins Univ. School of Hygiene and Public Health (United States)
Paul T. Strickland, Johns Hopkins Univ. School of Hygiene and Public Health (United States)
Richard C. Benson, Johns Hopkins Univ. (United States)

Published in SPIE Proceedings Vol. 3913:
In-Vitro Diagnostic Instrumentation
Gerald E. Cohn, Editor(s)

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