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Proceedings Paper

Multiphoton up-converting phosphors for use in rapid immunoassays
Author(s): R. Sam Niedbala; Timothy L. Vail; Hans Feindt; S. Li; Jarrett L. Burton
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Paper Abstract

There exists increasing demand for rapid in-vitro diagnostic test capable of detecting pathogens, disease markers, and drugs. Many currently marketed technologies are limiting due to their analytical sensitivity or the number of analytes detected in a single assay. Presented is the use of up- converting phosphors as reporters in rapid point-of-care diagnostic assays using a lateral flow format in a nitrocellulose matrix. Up-converting Phosphor Technology (UPT) utilizes lanthanide-doped ceramic particles that possess anti-Stokes shift emission. These material absorb multiple IR photons and emit dopant-dependent spectra in the visible range. Their signature spectra allow the development of sensitive, multi-analyte diagnostic test. Because no biological matrix up-converts in the IR, the sensitivity of the assay is enhanced, being determined by the affinity of the biological constituents and the level of non-specific binding of the particle reporter. Feasibility data presented below represent a wide range of molecular weight analytes, including drugs of abuse, proteins such as cardiac markers, and pathogenic microorganisms.

Paper Details

Date Published: 11 April 2000
PDF: 11 pages
Proc. SPIE 3913, In-Vitro Diagnostic Instrumentation, (11 April 2000); doi: 10.1117/12.382032
Show Author Affiliations
R. Sam Niedbala, STC Technologies, Inc. (United States)
Timothy L. Vail, STC Technologies, Inc. (United States)
Hans Feindt, STC Technologies, Inc. (United States)
S. Li, STC Technologies, Inc. (United States)
Jarrett L. Burton, STC Technologies, Inc. (United States)

Published in SPIE Proceedings Vol. 3913:
In-Vitro Diagnostic Instrumentation
Gerald E. Cohn, Editor(s)

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