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Proceedings Paper

Development of an automated handheld immunoaffinity fluorometric biosensor
Author(s): Terry E. Phillips; C. Brent Bargeron; Richard C. Benson; Micah A. Carlson; Allan B. Fraser; John D. Groopman; Harvey W. Ko; Paul T. Strickland; James Velky
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Paper Abstract

A new immunoaffinity fluorometric biosensor has been developed for detecting and quantifying aflatoxins, a family of potent fungi-produced carcinogens that are commonly found in a variety of agriculture products. They have also been cited as a biological agent under weapons development. The handheld, self-contained biosensor is fully automatic, highly sensitive, quick, quantitative, and requires no special storage. Concentration from 0.1 parts per billion to 50 ppb can be determined in less than 2 minutes with a 1 ml sample volume. Higher concentrations can be determined by simply reducing the sample volume. The device operates on the principles of immunoaffinity for specificity and fluorescence for a quantitative assay. The analytic procedure is flexible so that other chemical and biological analytes could be detected with minor modifications to the current device.

Paper Details

Date Published: 11 April 2000
PDF: 7 pages
Proc. SPIE 3913, In-Vitro Diagnostic Instrumentation, (11 April 2000); doi: 10.1117/12.382031
Show Author Affiliations
Terry E. Phillips, Johns Hopkins Univ. (United States)
C. Brent Bargeron, Johns Hopkins Univ. (United States)
Richard C. Benson, Johns Hopkins Univ. (United States)
Micah A. Carlson, Johns Hopkins Univ. (United States)
Allan B. Fraser, Johns Hopkins Univ. (United States)
John D. Groopman, Johns Hopkins Univ. School of Hygiene and Public Health (United States)
Harvey W. Ko, Johns Hopkins Univ. (United States)
Paul T. Strickland, Johns Hopkins Univ. School of Hygiene and Public Health (United States)
James Velky, Johns Hopkins Univ. (United States)

Published in SPIE Proceedings Vol. 3913:
In-Vitro Diagnostic Instrumentation
Gerald E. Cohn, Editor(s)

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