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Proceedings Paper

Next fundamental steps for optical DNA biosensors
Author(s): Lisa Henke; James H. Watterson; Ulrich J. Krull
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Paper Abstract

Methods for the detection of pathogenic organisms and genetic mutations have evolved to use the selective recognition chemistry offered by nucleic acid hybridization. The immobilization of single-stranded DNA as a 'receptor' onto various solid surfaces that are suitable for optical sensor development is a fundamental issue that determines aspects of selectivity, sensitivity and speed. This manuscript presents an evaluation of various immobilization strategies to attach ssDNA to surfaces, the control of such immobilization to design optical and thermodynamic properties of monolayer films, and suggests approaches for optimization of analytical figures of merit and the potential for quantitative analysis.

Paper Details

Date Published: 11 April 2000
PDF: 15 pages
Proc. SPIE 3913, In-Vitro Diagnostic Instrumentation, (11 April 2000);
Show Author Affiliations
Lisa Henke, Univ. of Toronto at Mississauga (Canada)
James H. Watterson, Univ. of Toronto at Mississauga (Canada)
Ulrich J. Krull, Univ. of Toronto at Mississauga (Canada)

Published in SPIE Proceedings Vol. 3913:
In-Vitro Diagnostic Instrumentation
Gerald E. Cohn, Editor(s)

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