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Proceedings Paper

Detection of salmonella using a real-time PCR based on molecular beacons
Author(s): Wilfred Chen; Grisselle Martinez; Ashok Mulchandani
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Paper Abstract

Molecular beacons are oligonucleotide probes that become fluorescent upon hybridization. We developed a new approach to detect the presence of Salmonella species using these fluorogenic reporter molecules and demonstrated their ability to discriminate between similar E. coli species in real-time PCR assays. A detection limit of 1 CFU per PCR reaction was obtained. The assays were carried out entirely in sealed PCR tubes, enabling fast and direct detection in a semiautomated format.

Paper Details

Date Published: 22 March 2000
PDF: 7 pages
Proc. SPIE 3926, Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing, (22 March 2000);
Show Author Affiliations
Wilfred Chen, Univ. of California/Riverside (United States)
Grisselle Martinez, Univ. of California/Riverside (United States)
Ashok Mulchandani, Univ. of California/Riverside (United States)


Published in SPIE Proceedings Vol. 3926:
Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing
Patrick A. Limbach; John C. Owicki; Ramesh Raghavachari; Weihong Tan, Editor(s)

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