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Proceedings Paper

Polyaniline-coated nanospray emitters: a route to low-flow separations mass spectrometry
Author(s): Troy D. Wood; Sarah A. Lorenz; E. Peter Maziarz III
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Paper Abstract

Nanoelectrospray or nanospray ionization has revolutionized biological mass spectrometry because of its low sample consumption requirements, low flow rates (nL/min), and improved ion transfer efficiency from source to detector versus conventional electrospray ionization. These advantages suggest that nanospray could prove beneficial in the coupling to on-line capillary separations methods, such as capillary electrophoresis and capillary electrochromatography. In order to establish a stable spray, the nanospray emitter must be electrically biased relative to a counter-electrode inlet to the mass spectrometer, this is most easily achieved by coating the nanospray emitter with a conductive metal coating. However, metallized nanospray tips are highly susceptible to deterioration by electrical discharge. Due to this limited lifetime, nanospray emitters are limited for coupling to capillary separations techniques. Here, an alterative coating for nanospray emitters using polyaniline is demonstrated.

Paper Details

Date Published: 22 March 2000
PDF: 8 pages
Proc. SPIE 3926, Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing, (22 March 2000); doi: 10.1117/12.380497
Show Author Affiliations
Troy D. Wood, SUNY/Buffalo and Roswell Park Cancer Institute (United States)
Sarah A. Lorenz, SUNY/Buffalo (United States)
E. Peter Maziarz III, Bausch & Lomb, Inc. (United States)

Published in SPIE Proceedings Vol. 3926:
Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing
Patrick A. Limbach; John C. Owicki; Ramesh Raghavachari; Weihong Tan, Editor(s)

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