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Proceedings Paper

Genotyping complex short tandem repeats using electrospray ionization Fourier transform ion cyclotron resonance multistage mass spectrometry
Author(s): James C. Hannis; David C. Muddiman; Allison P. Null
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Paper Abstract

Electrospray ionization Fourier transform ion cyclotron resonance (ESI-FTICR) mass spectrometry is a rapidly emerging, universal platform with the ability to provide detailed information regarding genetic variation and the up- and down regulation of their cognate gene products. Herein, we report our progress towards the development of ESI-FTICR mass spectrometry for the characterization of genomic regions which contain both a length and sequence polymorphism (i.e., complex short tandem repeats). Specifically, it is demonstrated for the first time that a high-quality ESI-FTICR mass spectrum of a 82-bp double- stranded PCR product derived from a single, 50 (mu) L PCR reaction with less than 10 X 10-15 moles injected into the mass spectrometer can be routinely obtained. It is important to note that each measurement, which translates to an accurate genotype, is completed on the timescale of seconds. Progress towards the implementation of flow- injection analysis methodology to increase the throughput is also presented using an alternating injection of a 15-mer and 16-mer oligonucleotide.

Paper Details

Date Published: 22 March 2000
PDF: 12 pages
Proc. SPIE 3926, Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing, (22 March 2000); doi: 10.1117/12.380492
Show Author Affiliations
James C. Hannis, Virginia Commonwealth Univ. (United States)
David C. Muddiman, Virginia Commonwealth Univ. and Medical College of Virginia (United States)
Allison P. Null, Virginia Commonwealth Univ. (United States)

Published in SPIE Proceedings Vol. 3926:
Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing
Patrick A. Limbach; John C. Owicki; Ramesh Raghavachari; Weihong Tan, Editor(s)

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