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Proceedings Paper

Design of a four-channel spectral analyzer to resolve linear combinations of two fluorescent spectra
Author(s): Gijsbert J. de Wolff; Lucas J. van Vilet
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Paper Abstract

Advances in molecular probes and fluorescence labeling require sophisticated spectral analysis. For the analysis of spectral data one frequently uses an RGB system (3D color system), dedicated filters for each n basic spectra (nD), or a spectral analyzer (mD, m>n). We present a method for designing spectral filters that optimally resolve all basic spectra as well as all linear combinations of two basic spectra.

Paper Details

Date Published: 14 March 2000
PDF: 11 pages
Proc. SPIE 3920, Spectral Imaging: Instrumentation, Applications, and Analysis, (14 March 2000); doi: 10.1117/12.379595
Show Author Affiliations
Gijsbert J. de Wolff, Delft Univ. of Technology (Netherlands)
Lucas J. van Vilet, Delft Univ. of Technology (Netherlands)

Published in SPIE Proceedings Vol. 3920:
Spectral Imaging: Instrumentation, Applications, and Analysis
Gregory H. Bearman; Dario Cabib; Richard M. Levenson, Editor(s)

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