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Proceedings Paper

Image reconstruction in tissuelike turbid media under single and two-photon excitation
Author(s): Xiasong Gan; N. Dragomir; Min Gu
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Paper Abstract

In this paper, we present the forward (optical gating) and inverse (reconstruction) approaches to obtain high-resolution imaging in tissue-like media under single-photon (1-p) and two-photon (2-p) excitation. Effective point spread functions (EPSF) for fluorescence microscopic imaging are introduced for fast image modeling and reconstruction. It is demonstrated that a deeper penetration depth can be achieved under 2-p excitation due to the use of a longer illumination wavelength and the non-linear dependence of the fluorescence on excitation intensity. The fundamental difference between 1-p and 2-p fluorescence imaging is that the penetration depth is limited by the degradation in image resolution in 1-p fluorescence, while the penetration depth is limited by the loss in signal strength in 2-p fluorescence imaging. Based on the EPSF that derived in the simulation, image reconstruction using deconvolution methods can partially recover the resolution loss.

Paper Details

Date Published: 15 March 2000
PDF: 7 pages
Proc. SPIE 3912, Micro- and Nanotechnology for Biomedical and Environmental Applications, (15 March 2000); doi: 10.1117/12.379581
Show Author Affiliations
Xiasong Gan, Swinburne Univ. of Technology (Australia)
N. Dragomir, Swinburne Univ. of Technology (Australia)
Min Gu, Swinburne Univ. of Technology (Australia)

Published in SPIE Proceedings Vol. 3912:
Micro- and Nanotechnology for Biomedical and Environmental Applications
Raymond P. Mariella Jr., Editor(s)

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