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Proceedings Paper

Micromachined pipettes integrated in a flow channel for single DNA molecule study by optical trapping
Author(s): Cristina R. Rusu; Ronny van't Oever; Meint J. de Boer; Henri V. Jansen; Erwin Berenschot; Miko C. Elwenspoek; Martin L. Bennink; Johannes Sake Kanger; Bart G. de Grooth; Jan Greve; Juergen P. Brugger; Albert van den Berg
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Paper Abstract

We have developed a micromachined flow cell consisting of a flow channel integrated with micropipettes. The flow cell is used in combination with an optical trap set-up (optical tweezers) to study mechanical and structural properties of (lambda) -DNA molecules. The flow cell was realized using silicon micromachining including the so-called buried channel technology to fabricate the micropipettes, the wet etching of glass to create the flow channel, and the powder blasting of glass to create the fluid connections. The volume of the flow cell is 2 (mu) l. The pipettes have a length of 130 micrometer, a width of 5 - 10 micrometer, a round opening of 1 micron and can be processed with different shapes. Using this flow cell we stretched single molecules ((lambda) -DNA) showing typical force-extension curves also found with conventional techniques.

Paper Details

Date Published: 15 March 2000
PDF: 9 pages
Proc. SPIE 3912, Micro- and Nanotechnology for Biomedical and Environmental Applications, (15 March 2000); doi: 10.1117/12.379579
Show Author Affiliations
Cristina R. Rusu, Univ. of Twente (Belgium)
Ronny van't Oever, Univ. of Twente (Netherlands)
Meint J. de Boer, Univ. of Twente (Netherlands)
Henri V. Jansen, Univ. of Twente (Belgium)
Erwin Berenschot, Univ. of Twente (Netherlands)
Miko C. Elwenspoek, Univ. of Twente (Netherlands)
Martin L. Bennink, Univ. of Twente (Netherlands)
Johannes Sake Kanger, Univ. of Twente (Netherlands)
Bart G. de Grooth, Univ. of Twente (Netherlands)
Jan Greve, Univ. of Twente (Netherlands)
Juergen P. Brugger, Univ. of Twente (Netherlands)
Albert van den Berg, Univ. of Twente (Netherlands)

Published in SPIE Proceedings Vol. 3912:
Micro- and Nanotechnology for Biomedical and Environmental Applications
Raymond P. Mariella Jr., Editor(s)

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