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Proceedings Paper

Multicolor fluorescence detection of ribosomal RNA in microchannels
Author(s): Michal Balberg; Krassimira Hristova; Margit Mau; Dominic Frigon; Henry Chris Zeringue; David J. Brady; David J. Beebe; Lutgarde Raskin
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Paper Abstract

A micro fluidic device capable of detecting the abundance of bacteria in an environmental solution is described. The micro channels are made of poly(dimethylsioxane) (PDMS) elastomer integrated with fused silica capillaries coated with Aluminum. The detection of specific bacteria is based on molecular probes (beacons) that emit a fluorescent signal only when hybridized to the target. This method allows hybridization in solution, without immobilization, and avoids washing of the unbound probes. By marking 16S rDNA oligonucleotide probes (different genetic sequences) with different color dyes, and detecting the spectral intensity of light in the micro- channel, different micro-organisms can be detected in one sample. Miniaturization of the analytic device allows the use of small quantities of RNA molecules, as target molecules, and improves the detection limits. Future devices should incorporate a parallel array of micro-channels, and enable fast and parallel processing of the molecular signals.

Paper Details

Date Published: 15 March 2000
PDF: 6 pages
Proc. SPIE 3912, Micro- and Nanotechnology for Biomedical and Environmental Applications, (15 March 2000); doi: 10.1117/12.379578
Show Author Affiliations
Michal Balberg, Univ. of Illinois/Urbana-Champaign (United States)
Krassimira Hristova, Univ. of Illinois/Urbana-Champaign (United States)
Margit Mau, Technische Univ. Freiberg (Germany)
Dominic Frigon, Univ. of Illinois/Urbana-Champaign (United States)
Henry Chris Zeringue, Univ. of Illinois/Urbana-Champaign (United States)
David J. Brady, Univ. of Illinois/Urbana-Champaign (United States)
David J. Beebe, Univ. of Illinois/Urbana-Champaign (United States)
Lutgarde Raskin, Univ. of Illinois/Urbana-Champaign (United States)

Published in SPIE Proceedings Vol. 3912:
Micro- and Nanotechnology for Biomedical and Environmental Applications
Raymond P. Mariella Jr., Editor(s)

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