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Proceedings Paper

Modular microinstrumentation for endothelial cell research
Author(s): Bonnie L. Gray; Abdul I. Barakat; Deborah K. Lieu; Scott D. Collins; Rosemary L. Smith
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Paper Abstract

Microfabrication technology is implemented to realize a fluidic microinstrument for the study of endothelial cell elongation and cell responsiveness to fluid flow. The microinstrument contains arrays of microchannels, 30 - 300 micrometer wide, that are fabricated by deep reactive ion etching (DRIE) of silicon and anodic bonding to glass. Silicon fluidic input/output modules, also micromachined in silicon, provide modular connections between the microchannels and off- chip devices for flow monitoring and control. Image analysis of cells cultured in microchannels shows that the cells become progressively more elongated as channel width decreases. When subjected to a fluid shear stress of 2 N/m2, cuboidal cells grown in 200 micrometer wide microchannels progressively align and elongate in the direction of flow.

Paper Details

Date Published: 15 March 2000
PDF: 7 pages
Proc. SPIE 3912, Micro- and Nanotechnology for Biomedical and Environmental Applications, (15 March 2000); doi: 10.1117/12.379565
Show Author Affiliations
Bonnie L. Gray, Univ. of California/Davis (Netherlands)
Abdul I. Barakat, Univ. of California/Davis (United States)
Deborah K. Lieu, Univ. of California/Davis (United States)
Scott D. Collins, Univ. of California/Davis (United States)
Rosemary L. Smith, Univ. of California/Davis (United States)

Published in SPIE Proceedings Vol. 3912:
Micro- and Nanotechnology for Biomedical and Environmental Applications
Raymond P. Mariella Jr., Editor(s)

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