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Proceedings Paper

Gene cloning of the 18S rRNA of an ancient viable moss from the permafrost of northeastern Siberia
Author(s): Damien Marsic; Richard B. Hoover; David A. Gilichinsky; Joseph D. Ng
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Paper Abstract

A moss plant dating as much as 40,000 years old was collected from the permafrost of the Kolyma Lowlands of Northeastern Siberia. The plant tissue was revived and cultured for the extraction of its genomic DNA. Using the polymerase chain reaction technique, the 18S ribosomal RNA gene was cloned and its sequence studied. Comparative sequence analysis of the cloned ribosomal DNA to other known 18S RNA showed very high sequence identity and was revealed to be closest to the moss specie, Aulacomnium turgidum. The results of this study also show the ability of biological organisms to rest dormant in deep frozen environments where they can be revived and cultured under favorable conditions. This is significant in the notion that celestial icy bodies can be media to preserve biological function and genetic material during long term storage or transport.

Paper Details

Date Published: 30 December 1999
PDF: 11 pages
Proc. SPIE 3755, Instruments, Methods, and Missions for Astrobiology II, (30 December 1999); doi: 10.1117/12.375074
Show Author Affiliations
Damien Marsic, Univ. of Alabama in Huntsville (United States)
Richard B. Hoover, NASA Marshall Space Flight Ctr. (United States)
David A. Gilichinsky, Institute of Photosynthesis and Soil Science (Russia)
Joseph D. Ng, Univ. of Alabama in Huntsville (United States)

Published in SPIE Proceedings Vol. 3755:
Instruments, Methods, and Missions for Astrobiology II
Richard B. Hoover, Editor(s)

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