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Proceedings Paper

Growth factors for nanobacteria
Author(s): Neva Ciftcioglu; E. Olavi Kajander
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Paper Abstract

Nanobacteria are novel microorganisms recently isolated from fetal bovine serum and blood of cows and humans. These coccoid, gram negative bacteria in alpha-2 subgroup of Proteobacteria grow slowly under mammalian cell culture conditions but not in common media for microbes. Now we have found two different kinds of culture supplement preparations that improve their growth and make them culturable in the classical sense. These are supernatant fractions of conditioned media obtained from 1 - 3 months old nanobacteria cultures and from about a 2 weeks old Bacillus species culture. Both improved multiplication and particle yields and the latter increased their resistance to gentamicin. Nanobacteria cultured with any of the methods shared similar immunological property, structure and protein pattern. The growth supporting factors were heat-stabile and nondialyzable, and dialysis improved the growth promoting action. Nanobacteria formed stony colonies in a bacteriological medium supplemented with the growth factors. This is an implication that nanobacterial growth is influenced by pre-existing bacterial flora.

Paper Details

Date Published: 30 December 1999
PDF: 7 pages
Proc. SPIE 3755, Instruments, Methods, and Missions for Astrobiology II, (30 December 1999); doi: 10.1117/12.375069
Show Author Affiliations
Neva Ciftcioglu, Univ. of Kuopio (Finland)
E. Olavi Kajander, Univ. of Kuopio (Finland)

Published in SPIE Proceedings Vol. 3755:
Instruments, Methods, and Missions for Astrobiology II
Richard B. Hoover, Editor(s)

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