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Proceedings Paper

Application and calibration of a field-portable excitation-emission matrix fluorometer for analysis of environmental contaminants
Author(s): Renee D. JiJi; Michelle L. Nahorniak; Evert Fruitman; Karl S. Booksh
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Paper Abstract

A field portable, single measurement, excitation-emission matrix fluorometer is applied to five environmental analyses. All applications require the uses of multi-way spectral deconvolution and calibration methods in order to uniquely resolve the spectral profiles of the targets analytes from interfering fluorophores. A modified multi-way calibration methods are applied to extend the dynamic range of the fluorometer by permitting robust calibration in the presence of intense Rayleigh and Raman backgrounds and localized detector saturation. The multi-way resolution methods are applied to calibrate chemically induced fluorescence changes induced by a target analyte from that of a fluorescent probe designed for molecular recognition. The multi-way resolution and calibration methods are also applied to potential sensor design relying of photoinduced degradation to and form fluorescent products. The EEM and multi-way methods are finally demonstrated in determining binding constants and capacity factors for fluorescent metal-ligand interactions.

Paper Details

Date Published: 16 December 1999
PDF: 10 pages
Proc. SPIE 3854, Pattern Recognition, Chemometrics, and Imaging for Optical Environmental Monitoring, (16 December 1999); doi: 10.1117/12.372888
Show Author Affiliations
Renee D. JiJi, Arizona State Univ. (United States)
Michelle L. Nahorniak, Arizona State Univ. (United States)
Evert Fruitman, Arizona State Univ. (United States)
Karl S. Booksh, Arizona State Univ. (United States)

Published in SPIE Proceedings Vol. 3854:
Pattern Recognition, Chemometrics, and Imaging for Optical Environmental Monitoring
Khalid J. Siddiqui; DeLyle Eastwood, Editor(s)

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