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Proceedings Paper

Assessment of inner filter effects in fluorescence spectroscopy using the dual-pathlength method: a study of the jet fuel JP-4
Author(s): Todd E. Pagano; Jonathan E. Kenny
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Paper Abstract

The integrity of most currently used fluorescence data analysis algorithms requires that a fluorophore present in a sample display the same spectral signature, apart from a single concentration-dependent scaling factor, regardless of its concentration or the concentrations of other components in the sample. Inner filter effects impede the utility of fluorescence spectroscopy by perturbing this spectral signature, rendering identification and quantification of fluorescent analytes difficult. The photophysical background theory of inner filtering is presented along with a comparison of existing correction schemes. The dual- pathlength method, a novel and simple experimental procedure for inner filter effect compensation, is introduced. The role of inner filtering in JP-4 a jet fuel contaminant detected by our laser-induced fluorescence/cone penetrometer instrument, is evaluated.

Paper Details

Date Published: 23 November 1999
PDF: 9 pages
Proc. SPIE 3856, Internal Standardization and Calibration Architectures for Chemical Sensors, (23 November 1999); doi: 10.1117/12.371303
Show Author Affiliations
Todd E. Pagano, Tufts Univ. (United States)
Jonathan E. Kenny, Tufts Univ. (United States)

Published in SPIE Proceedings Vol. 3856:
Internal Standardization and Calibration Architectures for Chemical Sensors
Ronald E. Shaffer; Radislav A. Potyrailo, Editor(s)

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