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Proceedings Paper

Novel approach to multichannel SPR sensing
Author(s): Jiri Homola; Hongbo B. Lu; Garet G. Nenninger; Sinclair S. Yee; Charles T. Campbell
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Paper Abstract

A novel approach to multichannel SPR sensing based on encoding information from separate sensing channels into a single optical spectrum is presented. A dual channel SPR sensor using this approach is demonstrated. Attention is given to exploitation of the dual-channel SPR sensor for compensation for background interference and non-specific adsorption of the biomolecules to the surface of the SPR biosensor. Experimental results indicate that background refractive index changes were compensated with accuracy better than 8 X 10-5 RIU (refractive index unit); the effect of a temperature change of 3.6 K was reduced by a factor of 13 by the dual-channel sensor. SPR biosensor-based detection of monoclonal anti-dinitrophenyl antibody (a-DNP) with compensation for non-specific adsorption is demonstrated.

Paper Details

Date Published: 18 November 1999
PDF: 9 pages
Proc. SPIE 3857, Chemical Microsensors and Applications II, (18 November 1999); doi: 10.1117/12.370287
Show Author Affiliations
Jiri Homola, Univ. of Washington (United States)
Hongbo B. Lu, Univ. of Washington (United States)
Garet G. Nenninger, Univ. of Washington (United States)
Sinclair S. Yee, Univ. of Washington (United States)
Charles T. Campbell, Univ. of Washington (United States)

Published in SPIE Proceedings Vol. 3857:
Chemical Microsensors and Applications II
Stephanus Buettgenbach, Editor(s)

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