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Proceedings Paper

Laser-induced microscopic autofluorescence imaging of human colonic tissues
Author(s): Zhiwei Huang; Teck-Chee Chia; Sing Lee; Wei Zheng; Sanjay M. Krishnan; Tuan-Kay Lim; Shusen Xie
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Paper Abstract

Using an excitation wavelength of 442 nm from a He-Cd laser, autofluorescence emissions from various layers of human colonic tissues were analyzed by a microspectrophotometric system. The cross section view on the sectioned colonic tissues reveals three distinct tissue layers for fluorescence, i.e., the mucosa, the submucosa and the muscularis propria, with submucosa being the most fluorescent. It was found that the acquired fluorescence imaging from different tissue layers could be used for localizing fluorescence as well as describing tissue architectural details of areas of distinct pathology that are essential for differentiating between normal and abnormal tissues by laser spectroscopic diagnostic approaches.

Paper Details

Date Published: 17 September 1999
PDF: 6 pages
Proc. SPIE 3863, 1999 International Conference on Biomedical Optics, (17 September 1999); doi: 10.1117/12.364405
Show Author Affiliations
Zhiwei Huang, Nanyang Technological Univ. (Canada)
Teck-Chee Chia, Nanyang Technological Univ. (Singapore)
Sing Lee, Nanyang Technological Univ. (Singapore)
Wei Zheng, Nanyang Technological Univ. (Singapore)
Sanjay M. Krishnan, Nanyang Technological Univ. (Singapore)
Tuan-Kay Lim, Nanyang Technological Univ. (Singapore)
Shusen Xie, Fujian Normal Univ. (China)

Published in SPIE Proceedings Vol. 3863:
1999 International Conference on Biomedical Optics
Qingming Luo; Britton Chance; Lihong V. Wang; Steven L. Jacques, Editor(s)

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