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Proceedings Paper

Optical biomolecular sensor based on surface plasmon resonance
Author(s): Yiwen Chen; Feng Qian; Hua Lu; Zu-Hong Lu
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Paper Abstract

A new optical bio-molecular sensors was developed for bio- molecular sensing based on light-excited surface plasmon resonance. Thickness of bio-molecule films absorbed onto a gold film is measured by the resonance condition of the surface plasmon on the sensing mental surface. The resonance condition is given by thickness of bio-molecule films faced on the mental. The developed sensor can be compact and simple, because of the absence of mechanical moving parts, by using a wedge of laser beam to obtain incident angle scanning and multi-channel angular light intensity detection with a charge coupled device. The sensor using Kretschmann configuration includes three parts: an optical measuring unit, a power source of laser, and a microcomputer used as data collection and handling. The adsorption of ferritin from phosphate buffered saline onto a gold film has been examined using the sensor. Application of the sensor allows elucidation of the surface layer thickness of dry ferritin on gold. Experiment results suggest less than monolayer coverage of ferritin on gold.

Paper Details

Date Published: 17 September 1999
PDF: 5 pages
Proc. SPIE 3863, 1999 International Conference on Biomedical Optics, (17 September 1999); doi: 10.1117/12.364358
Show Author Affiliations
Yiwen Chen, Southeast Univ. (China)
Feng Qian, Southeast Univ. (China)
Hua Lu, Southeast Univ. (China)
Zu-Hong Lu, Southeast Univ. (China)

Published in SPIE Proceedings Vol. 3863:
1999 International Conference on Biomedical Optics
Qingming Luo; Britton Chance; Lihong V. Wang; Steven L. Jacques, Editor(s)

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