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Proceedings Paper

Application of laser microbeam in biopharmacy
Author(s): Yansheng Zhao; Ming Wang; Luyan Wang; Taiming Li; Junmin Wu; Lei Xu; Xi Zhu
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Paper Abstract

Laser microbeam system consists of a third harmonic generation Nd:YAG laser and an inverted biological microscope. It is the first time that laser microbeam is applied in immobilized cell to produce midecamycin (MDM). After laser irradiation, the penetrance of immobilized granule greatly increased, probably through the microchannel made by laser. The results showed that the MDM productivity was increased greatly and the half-life of immobilized mycelia prolonged over three times as long as that of the control. In another experiment, plasmid pS65T (including green fluorescent protein genes) and plasmid pUH-10 (including Amp resistant gene) were successfully introduced into E. coli by laser microbeam. The results show that laser microbeam has a bright perspective on biopharmacy.

Paper Details

Date Published: 17 September 1999
PDF: 5 pages
Proc. SPIE 3863, 1999 International Conference on Biomedical Optics, (17 September 1999); doi: 10.1117/12.364349
Show Author Affiliations
Yansheng Zhao, China Pharmaceutical Univ. (China)
Ming Wang, China Pharmaceutical Univ. (China)
Luyan Wang, China Pharmaceutical Univ. (China)
Taiming Li, China Pharmaceutical Univ. (China)
Junmin Wu, China Pharmaceutical Univ. (China)
Lei Xu, China Pharmaceutical Univ. (China)
Xi Zhu, China Pharmaceutical Univ. (China)

Published in SPIE Proceedings Vol. 3863:
1999 International Conference on Biomedical Optics
Qingming Luo; Britton Chance; Lihong V. Wang; Steven L. Jacques, Editor(s)

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