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Proceedings Paper

Detection of trinitrotoluene (TNT) extracted from soil using a surface plasmon resonance (SPR)-based sensor platform
Author(s): Anita A. Strong; Donald I. Stimpson; Dwight U. Bartholomew; Thomas F. Jenkins; Jerome L. Elkind
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Paper Abstract

An antibody-based competition assay has been developed using a surface plasmon resonance (SPR) sensor platform for the detection of trinitrotoluene (TNT) in soil extract solutions. The objective of this work is to develop a sensor-based assay technology to use in the field for real- time detection of land mines. This immunoassay combines very simple bio-film attachment procedures and a low-cost SPR sensor design to detect TNT in soil extracts. The active bio-surface is a coating of bovine serum albumin that has been decorated with trinitrobenzene groups. A blind study on extracts from a large soil matrix was recently performed and result from this study will be presented. Potential interferant studied included 2,4-dinitrophenol, 2,4- dinitrotoluene, ammonium nitrate, and 2,4- dichlorophenoxyacetic acid. Cross-reactivity with dinitrotoluene will be discussed. Also, plans to reach sensitivity levels of 1ppb TNT in soil will be described.

Paper Details

Date Published: 2 August 1999
PDF: 11 pages
Proc. SPIE 3710, Detection and Remediation Technologies for Mines and Minelike Targets IV, (2 August 1999); doi: 10.1117/12.357059
Show Author Affiliations
Anita A. Strong, Texas Instruments Inc. (United States)
Donald I. Stimpson, Texas Instruments Inc. (United States)
Dwight U. Bartholomew, Texas Instruments Inc. (United States)
Thomas F. Jenkins, U.S. Army Cold Regions Research and Engineering Lab. (United States)
Jerome L. Elkind, Texas Instruments Inc. (United States)

Published in SPIE Proceedings Vol. 3710:
Detection and Remediation Technologies for Mines and Minelike Targets IV
Abinash C. Dubey; James F. Harvey; J. Thomas Broach; Regina E. Dugan, Editor(s)

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