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Proceedings Paper

Spectroscopic and time-sliced imaging of human breast tissue
Author(s): S. K. Gayen; M. E. Zevallos; Bidyut Baran Das; Robert R. Alfano
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Paper Abstract

Two-dimensional transillumination images of in vitro human breast tissue specimens were recorded as a function of the wavelength of near-infrared laser light, as well as, the position of picosecond-duration temporal slices of transmitted light pulse. Spectroscopic imaging arrangement made use of 1225 - 1300 nm light from a chromium-doped forsterite laser for sample illumination, a Fourier space gate and a polarization gate to sort out a fraction of the image-bearing photons, and an InGaAs area camera for recording two-dimensional images. Time-sliced imaging approach used 800-nm, approximately 130-fs duration, 1 kHz repetition-rate pulses from a Ti:sapphire laser system to illuminate the sample, and a gated imaging system that provided a variable-position, ~80 ps-duration electronic gate to record time-sliced two-dimensional images. Excised female breast tissue specimens comprising normal and cancerous, as well as, adipose and fibrous tissues were imaged. Marked enhancement of image contrast between adipose and fibrous tissues in the specimen was observed when the wavelength of imaging light was near-resonant with an absorption band of the human fatty tissue centered on 1203 nm. In time-sliced images, earlier time slices highlighted cancerous tissues, while the later slices accentuated the normal tissues in most of the specimens.

Paper Details

Date Published: 15 July 1999
PDF: 3 pages
Proc. SPIE 3597, Optical Tomography and Spectroscopy of Tissue III, (15 July 1999); doi: 10.1117/12.356851
Show Author Affiliations
S. K. Gayen, CUNY/City College (United States)
M. E. Zevallos, CUNY/City College (United States)
Bidyut Baran Das, CUNY/City College (United States)
Robert R. Alfano, CUNY/City College (United States)

Published in SPIE Proceedings Vol. 3597:
Optical Tomography and Spectroscopy of Tissue III
Britton Chance; Robert R. Alfano; Bruce J. Tromberg, Editor(s)

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