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Proceedings Paper

Detection and identification of single molecules in microcapillaries
Author(s): Christoph Zander; Karl-Heinz Drexhage
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Paper Abstract

A set-up permitting optical detection and identification of individual analyte molecules (dye labeled mononucleotides) in microcapillaries is described. It consists essentially of a confocal fluorescence microscope for time-correlated single-photon counting. Excitation of the analyte molecules takes place in the red spectral region with a short-pulsed diode laser at about 640 nm. A cone shaped capillary with an inner diameter at the very end of about 0.5 micrometers was used. The flow of the labeled nucleotides was established by electrokinetic forces. A maximum likelihood estimator was applied to analyze the measured fluorescence decays of the analyte molecules. This way two species of labeled mononucleotides can be distinguished by their characteristic fluorescence decay time on the single-molecule level.

Paper Details

Date Published: 19 July 1999
PDF: 2 pages
Proc. SPIE 3749, 18th Congress of the International Commission for Optics, (19 July 1999); doi: 10.1117/12.354800
Show Author Affiliations
Christoph Zander, Univ. of Siegen (Germany)
Karl-Heinz Drexhage, Univ. of Siegen (Germany)

Published in SPIE Proceedings Vol. 3749:
18th Congress of the International Commission for Optics
Alexander J. Glass; Joseph W. Goodman; Milton Chang; Arthur H. Guenther; Toshimitsu Asakura, Editor(s)

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