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Proceedings Paper

Classification of aortic atherosclerotic lesions with time-resolved fluorescence spectroscopy
Author(s): Jean-Michel I. Maarek; Laura Marcu; Warren S. Grundfest M.D.; Michael C. Fishbein M.D.
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Paper Abstract

In this study, we examine the possibility of differentiating between classes of atherosclerotic lesions based on time- resolved fluorescence spectroscopy and we compare the performance of classification schemes that use either the time-resolved spectra or only the intensity spectra. Transient fluorescence emissions induced by pulsed nitrogen laser excitation was measured on 87 excised samples of human aorta. The samples were classified histologically using the AHA classification Predictor variables derived from the time-resolved spectra included the spectral intensities at 360-510 nm and parameters of a biexponential fit of the fluorescence impulse response function. Stepwise discriminant analysis using these predict variables showed that a few predictor variables sufficed to correctly classify 89 percent of the samples. Excluding the time- dependent decay and using only the spectral intensities, the percentage of correctly classified cases was significantly lower: 51 percent. These results establish that time- resolved fluorescence spectroscopy markedly improved on the performance of steady-state fluorescence spectroscopy for fine classification of atherosclerotic lesions.

Paper Details

Date Published: 2 July 1999
PDF: 9 pages
Proc. SPIE 3600, Biomedical Imaging: Reporters, Dyes, and Instrumentation, (2 July 1999); doi: 10.1117/12.351030
Show Author Affiliations
Jean-Michel I. Maarek, Univ. of Southern California (United States)
Laura Marcu, Univ. of Southern California and Cedars-Sinai Medical Ctr. (United States)
Warren S. Grundfest M.D., Cedars-Sinai Medical Ctr. (United States)
Michael C. Fishbein M.D., Univ. of California/Los Angeles School of Medicine (United States)

Published in SPIE Proceedings Vol. 3600:
Biomedical Imaging: Reporters, Dyes, and Instrumentation
Eva Marie Sevick-Muraca; Darryl J. Bornhop; Christopher H. Contag; Eva Marie Sevick-Muraca, Editor(s)

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