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Proceedings Paper

Scanning apertureless fluorescence microscope
Author(s): Guillaume A. Lessard; T. Jason Yang; Pierre Barritault; Stephen R. Quake
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Paper Abstract

We describe a near-field apertureless fluorescence microscope, capable of imaging fluorescent latex beads with subwavelength precision. The instrument is based on a home- built tapping-mode atomic-force microscope, to which an inverted optical microscope was added. The fact that the wavelength of the fluorescence that we observe is different from the wavelength of the illumination allows for a relatively straightforward detection mechanism. Sample images are presented, along with evidence that the observe effect is of optical origin.

Paper Details

Date Published: 17 June 1999
PDF: 8 pages
Proc. SPIE 3607, Scanning and Force Microscopies for Biomedical Applications, (17 June 1999); doi: 10.1117/12.350630
Show Author Affiliations
Guillaume A. Lessard, California Institute of Technology (United States)
T. Jason Yang, California Institute of Technology (United States)
Pierre Barritault, California Institute of Technology (France)
Stephen R. Quake, California Institute of Technology (United States)

Published in SPIE Proceedings Vol. 3607:
Scanning and Force Microscopies for Biomedical Applications
Eiichi Tamiya; Shuming Nie, Editor(s)

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