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Proceedings Paper

Quantitative phase contrast microscopy of living cells by numerical reconstruction of digital holograms
Author(s): Etienne Cuche; Pierre Marquet; Pierre J. Magistretti; Christian D. Depeursinge
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Paper Abstract

We describe a new method of optical microscopy which allows simultaneously intensity and quantitative phase contrast imaging. The method is based on a new holographic imaging technique which involves a CCD camera as recording device and a numerical method for the reconstruction. An application to phase contrast imaging of living astrocytes is presented.

Paper Details

Date Published: 1 June 1999
PDF: 6 pages
Proc. SPIE 3604, Optical Diagnostics of Living Cells II, (1 June 1999); doi: 10.1117/12.349218
Show Author Affiliations
Etienne Cuche, Ecole Polytechnique Federale de Lausanne (Switzerland)
Pierre Marquet, Univ. de Lausanne (Switzerland)
Pierre J. Magistretti, Univ. de Lausanne (Switzerland)
Christian D. Depeursinge, Ecole Polytechnique Federale de Lausanne (Switzerland)

Published in SPIE Proceedings Vol. 3604:
Optical Diagnostics of Living Cells II
Daniel L. Farkas; Robert C. Leif; Bruce J. Tromberg, Editor(s)

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