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Proceedings Paper

Infrared spectroscopic studies of myeloid leukemia (ML-1) cells at different phases of the cell cycle
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Paper Abstract

Advances in infrared spectroscopic methodology permit excellent infrared spectra to be collected from objects as small as single human cells. These advances have lead to an increased interest of the use of infrared spectroscopy as a medical diagnostic tool. Infrared spectra of myeloid leukemia (ML-1) cells are reported for cells derived from an asynchronous, exponentially-growing culture, as well as for cells that were fractionated according to their stage within the cell division cycle. The observed results suggest that the cells' DNA is detectable by infrared spectroscopy mainly when the cell is in the S phase, during the replication of DNA. In the G1 and G2 phases, the DNA is so tightly packed in the nucleus that it appears opaque to infrared radiation. Consequently, the nucleic acid spectral contributions in the G1 and G2 phases would be mostly that of cytoplasmic RNA. These results suggest that infrared spectral changes observed earlier between normal and abnormal cells may have been due to different distributions of cells within the stages of the cell division cycle.

Paper Details

Date Published: 1 June 1999
PDF: 10 pages
Proc. SPIE 3604, Optical Diagnostics of Living Cells II, (1 June 1999); doi: 10.1117/12.349213
Show Author Affiliations
Susie Boydston-White, CUNY/Hunter College and CUNY/Graduate School (United States)
Max Diem, CUNY/Hunter College (United States)

Published in SPIE Proceedings Vol. 3604:
Optical Diagnostics of Living Cells II
Daniel L. Farkas; Robert C. Leif; Bruce J. Tromberg, Editor(s)

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