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Proceedings Paper

Three-dimensional reconstruction of active muscle cell segment volume from two-dimensional optical sections
Author(s): David S. Lake; P. J. Griffiths; G. Cecchi; Stuart R. Taylor
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Paper Abstract

An ultramicroscope coupled to a square-aspect-ratio sensor was used to image the dynamic geometry of live muscle cells. Skeletal muscle cells, dissected from frogs, were suspended in the optical axis and illuminated from one side by a focused slit of white light. The sensor detected light scattered at 90 degrees to the incident beam. Serial cross-sections were acquired as a motorized stage moved the cell through the slit of light. The axial force at right angles to the cross- sections was recorded simultaneously. Cross-sections were aligned by a least-squares fit of their centroids to a straight line, to correct for misalignments between the axes of the microscope, the stage, and the sensor. Three- dimensional volumes were reconstructed from each series and viewed from all directions to locate regions that remained at matching axial positions. The angle of the principal axis and the cross-sectional area were calculated and associated with force recorded concurrently. The cells adjusted their profile and volume to remain stable against turning as contractile force rose and fell, as predicted by the law of conservation of angular momentum.

Paper Details

Date Published: 1 June 1999
PDF: 13 pages
Proc. SPIE 3604, Optical Diagnostics of Living Cells II, (1 June 1999); doi: 10.1117/12.349192
Show Author Affiliations
David S. Lake, Mayo Foundation (United States)
P. J. Griffiths, Mayo Foundation (United States)
G. Cecchi, Mayo Foundation (United States)
Stuart R. Taylor, Mayo Foundation (United States)

Published in SPIE Proceedings Vol. 3604:
Optical Diagnostics of Living Cells II
Daniel L. Farkas; Robert C. Leif; Bruce J. Tromberg, Editor(s)

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