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Proceedings Paper

Hierarchical watershed transformation based on a-priori information for spot detection in 2D gel electrophoresis images
Author(s): Susan Wegner; Klaus-Peter Pleissner; Helmut Oswald; Eckart Fleck
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Paper Abstract

For the spot detection in 2D electrophoresis images an approach which is based on the combination of the watershed transformation (WST) with a-priori knowledge is presented. To identify spot regions in the over segmented result of the WST two types of regions have to be found: Regions that correspond to a complete spot and regions that cover only a part of a spot. The first localization step, the gray value analysis, is based on the assumptions that spot regions have significantly higher gray values than the background and that they border on a background region. Since not all remaining regions are spot or partial spot regions, additionally a curvature analysis is done. Here the a-priori knowledge is used that regarding a gel image as a surface, the shape of a spot is obviously convex. Consequently, considering the second derivative all required spot and partial spot regions can be obtained by the regions of convex curvature. In a final merging step all partial spot regions covering one spot have to be combined to only one spot region. As merging criterion two spot characteristics are used. A spot should have an approximately elliptical shape and partial spot regions of one spot should have a local convex curvature in a small neighborhood along their boundary.

Paper Details

Date Published: 21 May 1999
PDF: 12 pages
Proc. SPIE 3661, Medical Imaging 1999: Image Processing, (21 May 1999); doi: 10.1117/12.348632
Show Author Affiliations
Susan Wegner, German Heart Institute and Charite/Humboldt Univ. of Berlin (Germany)
Klaus-Peter Pleissner, Institute for Plant Genetics and Crop Plant Research (Germany)
Helmut Oswald, German Heart Institute and Charite/Humboldt Univ. of Berlin (Switzerland)
Eckart Fleck, German Heart Institute and Charite/Humboldt Univ. of Berlin (Germany)

Published in SPIE Proceedings Vol. 3661:
Medical Imaging 1999: Image Processing
Kenneth M. Hanson, Editor(s)

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