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Proceedings Paper

Three-dimensional imaging in microscopy
Author(s): S. Kessler; Rosemarie Hild; G. Nitzche; Jork Hebenstreit
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Paper Abstract

A theory of 3D- imaging of a thick object with a depth h (Fig.l ) is developed for vertical Illumination microscopy (Fig2). The amount as well as the phase of the Fourier spectrum which corresponds to the 3D- object is discussed in detaiL Fig3 shows the amount tT(01 of the Fourier spectrum for different object depth. ii (V I has zercs In the case of planar object (h0) . As the thickness of the object is increased an enhancement of the spatial frequencies and a shift of the Ninhia occurs. The zeri of the planar object spectrum are lsed.

Paper Details

Date Published: 1 July 1990
PDF: 2 pages
Proc. SPIE 1319, Optics in Complex Systems, (1 July 1990); doi: 10.1117/12.34812
Show Author Affiliations
S. Kessler, Friedrich-Schiller Univ. (Germany)
Rosemarie Hild, Friedrich-Schiller Univ. (Germany)
G. Nitzche, Friedrich-Schiller Univ. (Germany)
Jork Hebenstreit, Friedrich-Schiller Univ. (Germany)

Published in SPIE Proceedings Vol. 1319:
Optics in Complex Systems
F. Lanzl; H.-J. Preuss; G. Weigelt, Editor(s)

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