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Proceedings Paper

Confocal-line optical microscopy
Author(s): V. Evangelista; D. Guidarini; S. Vestri; Pier A. Benedetti
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Paper Abstract

Confocal optical microscopy offers major improvements and usefull investigative capabilities, over conventional microscopical imaging, expecially when high contrast and focal plane discrimination are required. Confocal operation implies space selective illumination and light detection. Potential uses can be both in the field of transmitted-light analysis of absorbing specimens or with incident-light configurations. Provisions for spatial scanning have to be included in the instrument and a variety of electromechanical solutions have been suggested by others, adaptable to incident-light work. Point illumination and detection is typical of proposed instruments and of their commercialy available versions, being principally suited to reflectance and fluorescence investigations. In current designs , intermediate deflection devices provide two dimensional scanning of the object, using a spot of concentrated light. The illuminated position of the object, is imaged back by the same optics on the spatial filter ( pin hole) placed in front of a photo-detecting device. Instruments of this type are of limited utility, in transmitted light work, since the scanning configuration doesn't permit to perform transmitted light confocal imaging. In fact an intrinsic limitation is due to the difficulty of designing and constructing a scanning device that guarants precise and stable correspondence between the position illuminated and the position detected which, in the case of transmission analysis reside on the opposite halves in the optical path, with respect to the specimen.

Paper Details

Date Published: 1 July 1990
PDF: 2 pages
Proc. SPIE 1319, Optics in Complex Systems, (1 July 1990); doi: 10.1117/12.34807
Show Author Affiliations
V. Evangelista, Institute of Biofisica CNR (Italy)
D. Guidarini, Institute of Biofisica CNR (Italy)
S. Vestri, Institute of Biofisica CNR (Italy)
Pier A. Benedetti, Institute of Biofisica CNR (Italy)

Published in SPIE Proceedings Vol. 1319:
Optics in Complex Systems
F. Lanzl; H.-J. Preuss; G. Weigelt, Editor(s)

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