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Proceedings Paper

Third-harmonic generation (THG) imaging: 3D contrast without absorption
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Paper Abstract

Recently a novel imaging technique based on third-harmonic generation (THG) was introduced. This technique relies on a third-order non-linear interaction to generate a coherent signal response on the third-harmonic frequency with respect to the fundamental input radiation. Here we report on the input NA dependence of the THG signal and examine the resulting imaging characteristics of this novel technique in terms of resolution and contrast generation. We'll demonstrate the potential of the technique through a number of imaging examples, with special emphasis on in vivo applications. The latter illustrates the non-invasive character of the technique.

Paper Details

Date Published: 6 May 1999
PDF: 9 pages
Proc. SPIE 3605, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI, (6 May 1999); doi: 10.1117/12.347556
Show Author Affiliations
Michiel Muller, Univ. of Amsterdam (Netherlands)
Jeffrey A. Squier, Univ. of California/San Diego (United States)
G. J. Brakenhoff, Univ. of Amsterdam (Netherlands)


Published in SPIE Proceedings Vol. 3605:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI
Dario Cabib; Carol J. Cogswell; Jose-Angel Conchello; Jeremy M. Lerner; Tony Wilson, Editor(s)

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