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Proceedings Paper

Simultaneous 2D analysis of multiple fluorescent probes using multispectral imaging microscopy
Author(s): Yashvinder S. Sabharwal; Mark F. Hopkins; Ronald M. Lynch; LaTanya Donaldson
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Paper Abstract

This research discusses the use of a new multispectral imaging system in fluorescence microscopy applications. We present the result of experiments designed to demonstrate the utility of this instrument for biological investigations. A phantom object was constructed using fluorescent microspheres and imaged to demonstrate the ability of the system to differentiate microscopic structures based on spectral properties. Excellent spectral isolation of subcellular structures, differentially labeled with fluorescent probes, was also obtained in images of neurons and rat sinusoidal epithelial cells demonstrating that separation of subcellular structures in biological imaging applications is possible with high spatial resolution and excellent signal to noise ratio. A final imaging experiment using this device with a confocal micro- endoscope shows the flexibility of the developed instrument when used with different imaging systems. In addition, the results indicate that multispectral investigations of tissue in-vivo may be possible.

Paper Details

Date Published: 21 April 1999
PDF: 7 pages
Proc. SPIE 3603, Systems and Technologies for Clinical Diagnostics and Drug Discovery II, (21 April 1999); doi: 10.1117/12.346733
Show Author Affiliations
Yashvinder S. Sabharwal, Optical Insights, LLC (United States)
Mark F. Hopkins, Optical Insights, LLC (United States)
Ronald M. Lynch, Health Sciences Ctr./Univ. of Arizona (United States)
LaTanya Donaldson, Health Sciences Ctr./Univ. of Arizona (United States)


Published in SPIE Proceedings Vol. 3603:
Systems and Technologies for Clinical Diagnostics and Drug Discovery II
Gerald E. Cohn; John C. Owicki, Editor(s)

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