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Proceedings Paper

Detection of prostaglandin H synthase (cyclooxygenase) -1 and -2 expression in single cancer cells by confocal Raman microspectroscopy
Author(s): Kasem Nithipatikom; Michael J. McCoy; Andre Kajdacsy-Balla; Sushma Kaul; Paul F. Lindholm; Sharon R. Hawi; Sunant Rochanakij; Fran Adar; William B. Campbell
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Paper Abstract

Prostaglandin H synthase (PGHS), commonly known as cyclooxygenase (COX), is the enzyme involved in the synthesis of biologically active prostanoids from arachidonic acid. There are two PGHS isozymes with identical functions, similar sizes and similar structures. PGHS-1 is constitutively expressed in most mammalian cells where as PGHS-2 is induced by various agents. PGHS-2 expression was increased with inflammation, mitogenesis, and some types of cancer. We have developed a method to simultaneously detect PGHS-1 and PGHS-2 in single cancer cells by using specific antibodies, surface-enhanced Raman scattering, and confocal Raman microspectroscopy. Cells were plated, cultured, incubated for immunocomplexation, and detected directly in the wells of a multiwell plate. The expression and localization of PGHS-1 and -2 in single malignant human hepatocytes was detected and compared to normal cells. Furthermore, the correlation between the expression of PGHS and the invasiveness of cancer cells was investigated in PC- 3 cell sublines.

Paper Details

Date Published: 9 April 1999
PDF: 8 pages
Proc. SPIE 3608, Biomedical Applications of Raman Spectroscopy, (9 April 1999); doi: 10.1117/12.345410
Show Author Affiliations
Kasem Nithipatikom, Medical College of Wisconsin (United States)
Michael J. McCoy, Medical College of Wisconsin (United States)
Andre Kajdacsy-Balla, Medical College of Wisconsin (United States)
Sushma Kaul, Medical College of Wisconsin (United States)
Paul F. Lindholm, Medical College of Wisconsin (United States)
Sharon R. Hawi, Medical College of Wisconsin (United States)
Sunant Rochanakij, Ramkhamhaeng Univ. (Thailand)
Fran Adar, Instruments SA, Inc. (United States)
William B. Campbell, Medical College of Wisconsin (United States)

Published in SPIE Proceedings Vol. 3608:
Biomedical Applications of Raman Spectroscopy
Michael D. Morris, Editor(s)

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