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Proceedings Paper

Detection of angiotensin II binding to single adrenal zona glomerulosa cells by confocal Raman microspectroscopy
Author(s): Michael J. McCoy; Timothy J. Habermann; Craig J. Hanke; Fran Adar; William B. Campbell; Kasem Nithipatikom
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Paper Abstract

We developed a confocal Raman microspectroscopic technique to study ligand-receptor bindings in single cells using Raman-labeled ligands and surface-enhanced Raman scattering (SERS). The adrenal zona glomerulosa (ZG) cells were used as a model in this study. ZG cells have a high density of angiotensin II (AII) receptors on the cellular membrane. There are two identified subtypes of AII receptors,namely AT1 and AT2 receptors. AII is a peptidic hormone, which upon binding to its receptors, stimulates the release of aldosterone from ZG cells. The cellular localization of these receptors subtypes was detected in single ZG cells by using immunocomplexation of receptors with specific antibodies and confocal Raman microspectroscopy. In the binding study, we used biotin-labeled AII to bind to its receptors in ZG cells. Then, avidin and Raman-labeled AII. The binding was measure directly on the single ZG cells. The results showed that the binding was displaced with unlabeled AII and specific AII antagonists. This is a rapid and sensitive technique for detection of cellular ligand bindings as well as antagonists screening in drug discovery.

Paper Details

Date Published: 9 April 1999
PDF: 9 pages
Proc. SPIE 3608, Biomedical Applications of Raman Spectroscopy, (9 April 1999); doi: 10.1117/12.345402
Show Author Affiliations
Michael J. McCoy, Medical College of Wisconsin (United States)
Timothy J. Habermann, Medical College of Wisconsin (United States)
Craig J. Hanke, Medical College of Wisconsin (United States)
Fran Adar, Instruments SA, Inc. (United States)
William B. Campbell, Medical College of Wisconsin (United States)
Kasem Nithipatikom, Medical College of Wisconsin (United States)

Published in SPIE Proceedings Vol. 3608:
Biomedical Applications of Raman Spectroscopy
Michael D. Morris, Editor(s)

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