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Proceedings Paper

Semiautomatic quality determination of 3D confocal microscope scans of neuronal cells denoised by 3D wavelet shrinkage
Author(s): Anca Dima; Michael Scholz; Klaus Obermayer
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Paper Abstract

The main goal of this work is to denoise 3D confocal microscope scans of neuronal cells taken at high resolution such that neuronal structures of size smaller than 1 micrometers become visible. Although scanning confocal microscopes give much clearer images than ordinary light microscopes do, the images are still noisy and blurred. Our goal is to filter out the noise in these images without disturbing the smallest neuronal structures which have the same signal amplitude and geometric size as the noise. In order to obtain a good scale-space representation of the analyzed image, we use the 3D-wavelet transformation. We extend the denoising method of Donoho for 3D data and obtain several ways of computing thresholds and noise variances. Finally we develop a quality measure, for images with tree like structures, to determine the denoising method and wavelet form best suited for a particular confocal scan.

Paper Details

Date Published: 22 March 1999
PDF: 12 pages
Proc. SPIE 3723, Wavelet Applications VI, (22 March 1999); doi: 10.1117/12.342957
Show Author Affiliations
Anca Dima, Technical Univ. of Berlin (Germany)
Michael Scholz, Technical Univ. of Berlin (Germany)
Klaus Obermayer, Technical Univ. of Berlin (Germany)

Published in SPIE Proceedings Vol. 3723:
Wavelet Applications VI
Harold H. Szu, Editor(s)

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